FastQCFastQC Report
Mon 20 Mar 2023
SRR6211772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36223034
Sequences flagged as poor quality0
Sequence length151
%GC20

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC1172850.0143.735612
ATAGCGA1175700.0143.387191
GGCTAAC1364050.0143.256962
AGCCATG1306500.0143.086551
GGCTTCA2412500.0142.808622
AGTTAGC1361450.0142.790392
GTCGTAG1879850.0142.743671
GCCATGC1312100.0142.475852
TCGTAGA1886950.0142.206562
AACAGGC2373300.0142.02742
GCCAAGA1114550.0141.667041
TCACGCA984150.0141.470582
AGCGACT424400.0141.106753
GATGTAC1079700.0141.016132
TGGAACA1176950.0140.758851
CCAAGAC1122300.0140.688752
ACAGGCG822900.0140.500183
TGGCTTC2457050.0140.21931
GTTAGCG463850.0140.217513
GTACGCA474150.0140.030561
Produced by FastQC (version 0.11.5)