FastQCFastQC Report
Mon 20 Mar 2023
SRR6211772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36223034
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12455283.4384971728210285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3033760.8375223345454718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2718950.7506135460657437No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA411420.11357966315024853No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAGCGACTAAGTGCATGT383370.10583597166377616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14183700.075.745861
GGTATCA14366150.074.1272668
CAGTGGT14386400.074.105834
TGGTATC14385300.073.886987
GTGGTAT14452700.073.768636
AGCAGTG14549800.073.337322
GTATCAA14632900.073.2663049
AGTGGTA14605350.073.0533145
GCAGTGG14930250.071.3152853
TCCGAAT207100.044.10636145
TGCGGTT162300.023.718477145
GCAGTTG275150.022.8063623
CATGGGG2376500.022.31005725-29
ATGGGGT593800.021.98651125-29
GAATGGG281250.021.265333145
CGATTAC104650.020.93816465-69
TTTCGGG97150.020.67029105-109
CATGGGT1032550.020.64835425-29
CACGGGA96350.020.164797145
GCGACTA80750.020.12897735-39
Produced by FastQC (version 0.11.5)