FastQCFastQC Report
Mon 20 Mar 2023
SRR6211771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52531022
Sequences flagged as poor quality0
Sequence length151
%GC22

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA23300100.0144.650862
CTGTCAG7713850.0144.44773
GTCTGTC23334800.0144.435761
CTGTCAT8530200.0144.328453
CTGTCAA6982350.0144.196123
ATGAATC3029000.0142.91812
TGTCAGT2857300.0142.569964
TGTCAGA2222000.0142.44094
AGTTAGC1526350.0142.359512
TGTCAAG2395050.0142.337924
TGTCATG2877250.0142.307114
GTACGCA984950.0142.205981
TAGCGAC814450.0142.191672
TGTCATA2638200.0142.140444
TGTCAGG2657000.0141.923264
TGTCAAT2574250.0141.847024
TATCAGC919900.0141.378221
ATAGCGA819350.0141.341311
ATCAGCA923500.0140.82712
TACGCAA994750.0140.8052
Produced by FastQC (version 0.11.5)