FastQCFastQC Report
Mon 20 Mar 2023
SRR6211770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29376496
Sequences flagged as poor quality0
Sequence length151
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAGT1824300.0142.945111
CGAAGTA1827800.0142.67142
CATCAAG1237900.0141.999071
CGACTGG1215300.0141.937471
GATCGCA837650.0141.356722
CCGTGAG1513150.0141.355271
ACCGAGA1014700.0141.333682
CGTGAGA1515650.0141.122122
AACCGAG1017050.0141.00711
CAAGCTA867300.0140.920942
GACTGGA1225350.0140.773332
AGATCGC842650.0140.517961
ACGTATC624300.0140.40481
AGCAGGA1379850.0140.394531
GTCACTA727900.0139.683212
ATGCCTA603800.0139.492661
CCGAGAT534350.0139.008583
AACGTGA725750.0139.008211
ACAAGCT879500.0138.966161
CAATGGA1654800.0138.889021
Produced by FastQC (version 0.11.5)