FastQCFastQC Report
Mon 20 Mar 2023
SRR6211770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29376496
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23679098.060556303243246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3642741.240018550885034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1355750.46150841135035303No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA785260.2673089397728034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA719550.24494071723189856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA443400.15093699398321705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT424270.14442498519905164No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA361200.1229554402948534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG318040.10826342256748389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT21501200.060.4412881
AGCAGTG21851450.059.1528242
CAGTGGT21822000.059.0159724
GGTATCA21807350.059.0099838
AGTGGTA21819350.058.995235
GTGGTAT21814650.058.9593286
GTATCAA22016400.058.7829259
TGGTATC21842200.058.739917
GCAGTGG22136800.058.2005543
CATGCGA71050.023.36783145
ATGGGGT616200.022.17416425-29
CGTGGGA98000.022.046371145
GGTATAA191000.021.4842628
TGGACGG155800.021.27509170-74
ATGGGGC390050.020.31653625-29
GGACGGG156350.020.21720970-74
GCAGTTG252200.020.0384543
CATGGGG2969650.019.91475525-29
TTAGGGC168400.019.70912645-49
TGGTATA213700.019.337837
Produced by FastQC (version 0.11.5)