FastQCFastQC Report
Mon 20 Mar 2023
SRR6211769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75326938
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3904140.5182926724035961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2265660.3007768615259524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1152640.15301830004028572No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC1110790.14746251865434912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA947040.12572394752060678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG846560.11238476200904383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800480.10626742852603407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG766180.10171394461832499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT56531700.0138.689511
AGCAGTG57723150.0135.789572
AGTGGTA56647450.0135.645135
CAGTGGT56940550.0135.408464
TGGTATC56667250.0135.332347
GTGGTAT56781850.0135.305956
GGTATCA56391950.0134.620628
GTATCAA56526250.0134.24639
GCAGTGG58892400.0133.060413
GTATCGA442400.0130.742439
GGTATCG446250.0129.969218
GTGGGAT1205450.097.487126
CAGTGGG1294550.091.604194
GGATCAA1433100.079.134059
AGTGGTC362400.069.218615
AGTGGGA1838300.064.106445
GTATCCA436700.056.650849
GGGATCA2099200.054.1027578
GTGGTCT472000.053.023856
GGTATCC505250.048.89358
Produced by FastQC (version 0.11.5)