FastQCFastQC Report
Mon 20 Mar 2023
SRR6211768_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211768_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73046048
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC4592600.0142.396421
GAGCTGA4960350.0141.873551
ACGCTTA4988350.0141.671862
ACTTCGA4833700.0141.57812
CGTGAGA8457600.0141.149522
AGCTGAA4989500.0141.044682
CAAGCTA6626950.0140.699692
ATAGACA4649750.0140.646242
CTCAATG5288600.0140.554111
ACAAGCT6686200.0139.452881
CCGTGAG8563200.0139.408871
TCAATGA5351000.0138.915052
ACTAGTA2718750.0138.761522
CACTTCG4932650.0138.7381
GACTAGT2726900.0138.34681
ATGCCTA2556700.0138.215671
ACGTATC1363800.0138.079031
AACGCTT5126300.0137.859421
CGCTTAG1710800.0137.583343
CAGTTCA3087200.0137.564962
Produced by FastQC (version 0.11.5)