FastQCFastQC Report
Mon 20 Mar 2023
SRR6211764_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211764_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79665234
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24073133.0217861407398865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8322661.0447041428385186No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA6463180.8112924139531179No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5415350.6797632703871804No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA3778770.47433112416389817No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3673320.4610944844522769No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT1419850.17822705447648593No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1307790.1641606927307839No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC992080.12453110976866019No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA968930.12162519977032893No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA900080.1129827849372789Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC2264450.0140.16721
GTCGTAG2496750.0139.56911
ATAGCGA1495800.0139.553731
AGTTAGC1726850.0139.461042
TCGTAGA2478250.0138.94352
TAGCGAC1516100.0138.274672
TGGTCAG3796850.0137.789121
GATAGAC1401500.0137.592711
ATCAGCA2276200.0137.47942
GGCTAAC1529850.0137.314332
GGTCAGT3509200.0136.329852
TAGGATG2171650.0135.972931
CAGTGAC2831350.0135.910425
TCTGTCA2211400.0135.828462
AGGCTAA1530950.0135.752211
TCAGTGA2444200.0135.728874
GTCAGTG3411750.0135.672333
TCACGCA1340650.0135.646512
ATAGACA1429150.0135.329282
AGGATGA2140950.0135.252432
Produced by FastQC (version 0.11.5)