FastQCFastQC Report
Mon 20 Mar 2023
SRR6211763_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211763_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72354363
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5986620.8274027649168856No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4353110.6016375266823923No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3222180.4453331998790453No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2266160.3132029508711175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2279550.0140.966831
TAGCGAC2287700.0140.411222
TATCAGC1754300.0139.180041
CTCAATG2413050.0138.930761
CGTGATG3761450.0138.728621
GATAGAC1363350.0137.404221
ACTTCGA1074100.0137.303422
CGAACTT1191550.0137.210711
ATGTGAC2723200.0137.13715
ATCAGCA1751400.0137.062762
AGCTGAA1689750.0136.77882
CCGAAGT1438950.0136.591171
CGAGTAA1559100.0136.383772
AATCCGT1070150.0135.96631
AGGCTAA1048550.0135.916981
CTAACGA1887350.0135.90852
GCGAGTA1587450.0135.844271
TCAATGA2423800.0135.830812
ATCCGTC1073400.0135.058242
TGGAACA2926350.0134.976911
Produced by FastQC (version 0.11.5)