FastQCFastQC Report
Mon 20 Mar 2023
SRR6211762_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211762_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81812546
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21092952.5782048146992027No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15483451.8925520298561544No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10928151.3357547875358873No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA4292160.5246334712526853No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2691590.328994773002175No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT2351280.2873984633114828No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1977210.24167564715563308No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT997200.1218883959435757No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC931870.11390306811867217No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC927230.11333591794099648No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT914000.11171880655077035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA4106600.0141.48042
ATGCCTA2546750.0140.8341
TGCCTAA2536200.0139.623292
ACCTTAC3079400.0138.803392
AGTTAGC1202450.0137.136812
CACCTTA3088550.0136.88741
GCCTAAT1163750.0136.23613
AGAGTCA1145700.0136.184811
AGTGGTC2523500.0135.667771
CAAGCTA1104250.0135.219452
GTGGTCA2512650.0135.024892
GAGTCAA1139050.0134.951232
CAATGGA3311700.0134.948361
ATCGGTG3579000.0134.700643
AACGCTT4378350.0134.428671
CATCGGT3547850.0134.376342
CGCTTAA1308650.0134.341193
ATGAATC1331350.0134.266622
CCTAATC1252200.0133.64291
ACGTATC1020100.0133.002241
Produced by FastQC (version 0.11.5)