FastQCFastQC Report
Mon 20 Mar 2023
SRR6211761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83968590
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6608620.7870347709780525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3138000.37371116985529945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1139080.1356554873673596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1126120.13411205309032817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT41194600.0140.944121
TGGTATC41346100.0139.951867
AGTGGTA41460300.0139.943195
AGCAGTG41470650.0139.791032
GTGGTAT41508000.0139.735726
CAGTGGT41539100.0139.606314
GGTATCA41573550.0139.274668
GTATCAA41868300.0138.605779
GCAGTGG42823050.0135.323723
TGGTATA408100.043.223227
TAGCGAC34050.043.1697652
GGTATAA432250.040.6917238
GTTGTAT354550.038.0548256
GCAGTTG380250.035.2459753
TTGTATC386300.035.0202837
CAGTTGT416500.032.6020054
CAACGCA41540650.028.27597210-14
ATCAACG41553500.028.20793710-14
TCAACGC41610950.028.19145210-14
CGCAGAG41530800.028.18143815-19
Produced by FastQC (version 0.11.5)