FastQCFastQC Report
Mon 20 Mar 2023
SRR6211759_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211759_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68209834
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC5264700.0143.561941
ATCAGCA2694200.0143.53732
TATCAGC2695300.0143.478731
AACTCAC2591850.0143.365771
ATAGCGA2274100.0143.281891
ACTCACC2593600.0143.269032
TAGCGAC2275100.0143.218922
TCATTCC5279500.0143.15952
CCTAATC1693800.0143.131771
TCACGCA2391800.0143.127882
ACCTTAC1742650.0143.06782
CTTCACA3401100.0143.010152
CTAATCC1697200.0142.845032
CGACAAC1987750.0142.600052
CAGATTC1914150.0142.589772
CCGACAA1989750.0142.456711
GATGTAC2603650.0142.434072
CACGCAT872300.0142.21073
TCTTCAC3421750.0142.147081
TCAGCAT957500.0142.06423
Produced by FastQC (version 0.11.5)