FastQCFastQC Report
Mon 20 Mar 2023
SRR6211758_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211758_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72718603
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA35917300.0143.735862
TGCGAAT13773800.0143.456883
TGCGAAA10216250.0143.158923
TGCGAAG11953150.0143.08433
GCGAATA3995350.0142.89764
GGTGCGA36183650.0142.677811
TACGCAA1355950.0142.66412
TCGTAGA3838500.0142.496292
GTACGCA1358200.0142.427751
GCGAATG4809700.0142.395134
ATAGCGA1059800.0142.203341
GCGAAAT3749100.0142.132134
TAGCGAC1060450.0142.11622
GCGAAGA3262650.0141.997534
GCGAAGT4347300.0141.78094
GCGAATT5040250.0141.719864
GTCGTAG3867850.0141.415011
GCGAAAG3643300.0141.31124
CCTAATC1130800.0141.182621
AGAGATC1708700.0140.7652
Produced by FastQC (version 0.11.5)