FastQCFastQC Report
Mon 20 Mar 2023
SRR6211756_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211756_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51394356
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC1550550.0141.452261
ACGCTTA1645050.0141.157582
ACTTCGA1344850.0141.051062
CTCAATG2277450.0140.82161
GATAGAC2053300.0140.49351
CGTGAGA3098400.0140.400942
ACGTGAT2123300.0140.341312
GAGCTGA2148300.0139.980361
CCGTGAG3108300.0139.953771
AGCTGAA2159750.0139.238252
CGAAGTA1960200.0139.137042
ACTAGTA2043750.0138.96492
AACGTGA2146350.0138.834171
CCGAAGT1967450.0138.624341
CGTATCA1582250.0138.618292
CAAGCTA1729600.0138.612172
GACTAGT2050750.0138.490591
CATACCA2114450.0137.84641
CACTTCG1376550.0137.802861
CGAACTT1222750.0137.378881
Produced by FastQC (version 0.11.5)