FastQCFastQC Report
Mon 20 Mar 2023
SRR6211755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55797875
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3427100.0142.889451
TGCCTAA3459900.0141.534872
ACGCTTA1788700.0141.307952
AACATCG2783150.0141.105742
CATACCA3852500.0140.41751
GATCGCA1503500.0140.12212
TCCTGTA3376000.0140.081042
CTGGCAT1643100.0139.56961
ATCCTGT3395950.0139.258121
AGATCGC1512850.0139.256091
GTCACTA1082350.0138.789782
ACTTCGA965650.0138.565732
CCGAAGT1893350.0138.375661
CGAAGTA1893500.0138.364692
CAGTTCA1650700.0138.219922
TGGCATA1659700.0138.173662
AGTCACT1089250.0137.910581
ACGTGAT1343950.0137.337692
GCCTAAG1325700.0136.871383
ATACCAA3956100.0136.740332
Produced by FastQC (version 0.11.5)