FastQCFastQC Report
Mon 20 Mar 2023
SRR6211752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63470696
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10263151.6169903036828206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9135351.4393020048180973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1933550.3046366468078434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1142800.18005159420341002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA913090.14386008938676204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT911950.14368047894102184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA776660.12236513051629369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT31401800.0123.1414641
AGTGGTA31943250.0120.616325
GGTATCA31857550.0120.5402768
GTGGTAT31975350.0120.486396
AGCAGTG31997450.0120.443732
TGGTATC31909700.0120.287857
GTATCAA32047250.0120.1878059
CAGTGGT32123000.0119.915094
GCAGTGG32617650.0118.081923
GGTATAA354200.039.461538
TGGTATA381900.036.6372647
CGGTCTG130100.029.984798145
CGCATAC59550.028.1538561
CATGGGG10027450.025.96612725-29
GCAGTTG473400.025.5701123
ATGGGGG3304750.025.42939625-29
ACATGGG30501600.024.9672920-24
AGAGTAC31890750.024.75897415-19
TACATGG31577500.024.73610920-24
CAGAGTA31916600.024.73210515-19
Produced by FastQC (version 0.11.5)