FastQCFastQC Report
Mon 20 Mar 2023
SRR6211751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56274650
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5995801.0654530947771332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG564480.1003080427865833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT24153900.0141.266921
TGGTATC24459800.0138.763447
GTGGTAT24511650.0138.763326
AGTGGTA24531650.0138.668215
AGCAGTG24540900.0138.644522
GGTATCA24509850.0138.52098
CAGTGGT24630850.0138.073444
GTATCAA24664600.0138.013929
GCAGTGG26144450.0130.088993
TGGTATA259000.034.485047
GGTATAA262500.033.9423838
GCAGTTG309350.029.0911883
CGGTCTG80950.028.575556145
CAACGCA24487650.028.48074310-14
GTTGTAT317900.028.4149766
ATCAACG24500400.028.36348310-14
TCAACGC24601400.028.34581610-14
AACGCAG24627400.028.31394810-14
CGCAGAG24483600.028.30789815-19
AGAGTAC24658950.028.2218515-19
Produced by FastQC (version 0.11.5)