FastQCFastQC Report
Mon 20 Mar 2023
SRR6211750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58411209
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC4414950.0142.619671
AACATCG4206950.0142.035282
CTGGCAT4108900.0141.984091
TGGCATA4118350.0141.658282
GACTAGT3294150.0141.571911
ACTTCGA1890600.0141.078982
ACTAGTA3313450.0140.74732
CTCAATG2490050.0140.327031
GCATACA4492900.0140.145282
TGAGCCA4431050.0139.514772
CTGAGCC4446800.0139.020631
CAAGCTA2486100.0138.879072
CATACCA3154700.0138.580231
ACAAGCT2516450.0137.20411
GCCACAT2635150.0137.186341
CACTTCG1946000.0137.062671
CGAAGTA1720250.0137.016392
TCAATGA2554900.0136.765172
ACATCGT1575550.0136.730043
GTCACTA1093650.0136.091492
Produced by FastQC (version 0.11.5)