FastQCFastQC Report
Mon 20 Mar 2023
SRR6211749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96010485
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19906702.0733881304734583No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8460050.8811589692521602No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1727420.1799199327031834No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1062060.11061916831271086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTA5232650.0140.914892
CTGGCAT4445750.0140.455491
GTCACTA3672350.0140.36372
TGGCATA4434950.0140.287172
AGCTGAA3420850.0140.116172
AAGACTA2986100.0139.545882
CAAGACT2996750.0139.396041
GAGCTGA3448500.0139.336211
CGAACTT2039200.0138.833481
GAACTTA2035800.0138.703982
CAAGCTA1787850.0138.647842
GGCATAT2156250.0137.823963
GAGTCAA1364850.0137.611742
AGAGTCA1374450.0137.59091
ACTTCGA1360700.0137.274692
CAATGGA3254850.0137.23371
AGTCACT3774750.0137.004781
AGACTAT1497900.0136.980533
CCGAAGT5412450.0136.4761
ACAAGCT1826600.0136.308321
Produced by FastQC (version 0.11.5)