FastQCFastQC Report
Mon 20 Mar 2023
SRR6211747_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211747_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93277962
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17355711.8606442109016061No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12195911.3074803242377873No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT3013830.32310204204504384No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2771870.29716236724811806No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2030390.2176709220984052No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC973180.1043311816782618No Hit
CTGACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT942370.10102815067936412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT2418800.0140.06871
ACGTATC2346800.0139.740551
AGTCACT2489900.0139.557281
GTCACTA2492700.0139.365892
GAACTTA2418650.0139.333112
AACATCG3071850.0139.233342
CTCAATG2933550.0138.766421
AAGACTA2365050.0138.678452
ACGCTTA1779250.0138.505682
CAAGACT2379350.0138.298431
AGAGTCA1620500.0138.21921
GAGTCAA1612350.0138.174552
CCGAAGT1834300.0137.983951
ACTTCGA1422650.0137.823412
CGAAGTA1840300.0137.276372
CAATGGA3005850.0136.522111
AGACTAT1168600.0136.162553
TGAGCCA2151450.0135.974232
TCAATGA2977850.0135.807222
AACGTGA1457850.0135.287321
Produced by FastQC (version 0.11.5)