FastQCFastQC Report
Mon 20 Mar 2023
SRR6211746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121008875
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2328736719.24434633410153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20571411.6999918394415285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG7335730.6062142136268931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACTATAGGCCCCTTATATT7232300.5976669066628377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA6634730.5482845783005585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5666710.4682887928674653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTACCAACAACAACAAAC4085790.337643829843059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCCTAAAAAAAAACCAAC4066900.33608278731621954No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4022760.3324351209776969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCGGGTGCCACTCGGCA3461410.2860459615048896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA3245430.26819768384756904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA2707160.22371582249649044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2388820.19740866114158984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTATAGGAAAAAATAAA2297130.1898315309517587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA2229720.18426086516381546No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2117030.17494832507119829No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2104610.17392195407155053No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2047030.16916362539524477No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2022550.167140633279997No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1653840.13667096731541387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1645190.1359561437125996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1638410.13539585422970007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1581560.13069785170715784No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1560370.12894674047667992No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1517100.12537096969127265No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1485180.12273314663903785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGAAGCGTCCTCAGCGGC1395140.11529237008442562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTAAAAAAAAACCAACC1297920.10725824862019419No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1259740.10410310813979554No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1250430.1033337430828937No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1222610.10103473815453619No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCGGGTGCCACTCGGC1210980.10007365162265991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT170667500.063.7206461
AGCAGTG175590950.061.9530562
AGTGGTA175378900.061.8594325
GTATCAA175602100.061.8444759
CAGTGGT177273700.061.1975174
GGTATCA177586200.061.1374558
GCAGTGG178933750.060.795023
TGGTATC179505100.060.4813737
GTGGTAT179525450.060.427726
ATGGGGC4569200.022.91326925-29
ATGGGGG6548900.022.63212425-29
ACACTCG583250.022.40933630-34
TTACCCG1265850.022.10727550-54
ATGGGGT3924700.021.57333625-29
TCAGGCG1307950.021.55396540-44
GCGACCC1269100.021.5280430-34
CGACCCC1283950.021.43269330-34
TACCCGC1355050.021.22165350-54
CGGGATT1316150.021.20046845-49
TGGAATC746300.021.0972067
Produced by FastQC (version 0.11.5)