FastQCFastQC Report
Mon 20 Mar 2023
SRR6211745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133346865
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG7527600.0141.576322
TAGCGAC3177400.0141.134982
ACATCGT3120850.0140.695653
ATAGCGA3209100.0139.740831
CTGGCAT6222700.0139.552891
TGGCATA6268450.0138.534362
ACATCGA2147350.0138.165123
GTGCGAA5665100.0137.465072
ACATCGG2389050.0137.314163
GTGGTCA4803800.0136.994672
ATCCGTC2798050.0136.849382
ATGCCTA2913100.0136.716281
TATCAGC2284250.0135.951891
ATCAGCA2285950.0135.85082
AGTGGTC4846850.0135.777881
AAACATC7870850.0135.402151
GGCATAG2098100.0134.957153
AGCACCT4718750.0134.793531
AGTACAA4072950.0134.482221
AGCGACT1251000.0134.128043
Produced by FastQC (version 0.11.5)