FastQCFastQC Report
Mon 20 Mar 2023
SRR6211744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136973923
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC5193950.0140.650512
TAGCGAC2206200.0139.753422
GATAGAC2800850.0139.506971
ACGTATC1985500.0138.502611
ATAGCGA2236850.0137.838471
GCGAGTA3003700.0137.765341
CGAGTAA3015950.0137.205762
ACGCTTA3550850.0136.812872
CAGATTC5139850.0136.79412
GATCGCA1687850.0136.412612
ATAGACA2870400.0136.126712
ACCGAGA3671250.0135.604982
GGCTTCA7411950.0135.449462
GGCTAAC3920200.0135.122012
ACTAGTA2708850.0134.722062
ACACGAC2639650.0134.604491
TGGCTTC7461000.0134.558991
CGTATCA2048400.0134.249622
AGTCACT1557450.0134.247861
GTCACTA1557450.0134.247862
Produced by FastQC (version 0.11.5)