FastQCFastQC Report
Mon 20 Mar 2023
SRR6211744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136973923
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15157791.1066186663865938No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13345670.9743219517776387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10095810.7370607323556032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8356140.6100533457014297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT6439910.4701559142757414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC6133790.4478071347930949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT5617350.41010360782322053No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4693170.3426323709805698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA4460690.3256597973031699No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3986190.29101816701270944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT3571180.2607196991795292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT3524650.25732270221975023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC3468790.2532445537096868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCTTTAATTTATTAATG3316750.24214463069733355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC3198810.23353423264368356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA3090110.22559841554658544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTCGTTTGTTCAACGAT2562700.1870940062072983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG2538700.18534184787859218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC2260390.1650233818593339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG1992660.14547732563664692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1807450.13195577380082776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1752900.1279732639328728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT1620820.11833055259722686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAGGTTTGGTCCTAGCCT1469250.10726494268547744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCTTAGTTCAACTTTA1455460.10625818171244171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT74333200.0133.083851
GTGGTAT75834100.0130.184086
CAGTGGT76113600.0129.896824
GGTATCA75861950.0129.791788
AGTGGTA76090550.0129.77545
AGCAGTG76201450.0129.769582
TGGTATC75955450.0129.630317
GTATCAA76237400.0129.18119
GCAGTGG77030600.0128.341693
GGTATGA601250.036.92348
TGGTATG671700.033.156347
CAACGCA75782550.026.7088310-14
CATGGGG29703300.026.59591125-29
ATCAACG75876200.026.55879210-14
ACATGGG74943000.026.55647320-24
TCAACGC76140200.026.54411110-14
CGCAGAG76261100.026.50639515-19
TACATGG76027700.026.49637820-24
CAGAGTA76563800.026.44308715-19
AACGCAG76775050.026.43044310-14
Produced by FastQC (version 0.11.5)