FastQCFastQC Report
Mon 20 Mar 2023
SRR6211742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115877554
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA18448000.0142.920991
CGAGTAA18519050.0142.335742
ATCAGCA3688550.0139.464432
TATCAGC3696200.0139.30821
ATAGCGA1360850.0138.443241
AGGCTAA3588800.0137.580721
TAGCGAC1367650.0137.481232
GTGCGAA2342150.0137.344242
GGCTAAC3600500.0137.103652
GAGTAAG6885800.0137.045243
CAGATTC3946850.0135.769932
ACACGAC1846500.0135.581541
GATAGAC1171300.0135.314541
CCGTCTA5022100.0135.143162
GACTAGT1774800.0134.979681
GAGTAAT7093150.0134.638293
AGATTCG1320450.0134.435233
TCGTAGA1703450.0134.392492
TGGAACA4446250.0133.990621
ATCCGTC1677800.0133.705782
Produced by FastQC (version 0.11.5)