FastQCFastQC Report
Mon 20 Mar 2023
SRR6211740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81632636
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC5437350.0142.54791
ATAGACA5492550.0141.115312
AACGTGA3196600.0140.811551
ACGTGAT3197700.0140.76312
CGAAGTA2371050.0140.617812
GTGGTCA4057500.0139.502912
ACGTATC1606050.0139.455581
AGGTACA3260300.0139.146162
ATGCCTA2172750.0139.09921
CGCTGAT1373900.0138.860981
CCGAAGT2407450.0138.49171
ACGCTTA1412950.0138.475862
GATCGCA1951250.0138.454182
CTGGCAT2814750.0138.326541
AAGGTAC3279950.0138.312561
TGGCATA2824200.0137.86372
GTCACTA1076500.0137.688262
GCTGATC1387400.0137.50982
TAGACAT2000100.0137.472083
CGTGATG1458800.0137.423283
Produced by FastQC (version 0.11.5)