FastQCFastQC Report
Mon 20 Mar 2023
SRR6211738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115072334
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA8716150.0142.705141
ACGTGAT8717800.0142.678132
ACGTATC7555100.0142.459751
CGACTGG6234450.0142.246341
GACTGGA6250050.0141.89132
CGCTGAT3594550.0141.059561
GCTGATC3601350.0140.793212
GATAGAC2963650.0140.739151
ACCGAGA3824200.0140.638152
GTGGTCA6845250.0140.538482
CGTGATG3914400.0140.476073
AACATCG6103450.0140.23822
CGCATAC3274350.0140.1761
GCATACA3275000.0140.148162
ACGCTTA2726200.0140.116272
CGTGATA2169800.0139.434173
GCCACAT5243000.0139.226011
ATGCCTA2996850.0138.723591
ATAGACA3013450.0138.41332
TGAGCCA4733300.0138.235052
Produced by FastQC (version 0.11.5)