FastQCFastQC Report
Mon 20 Mar 2023
SRR6211738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115072334
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9476920.8235619866717921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4952440.43037625360062653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4152870.36089213242168183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2556050.2221255023818323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2470250.21466932268880545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2300100.19988297100152677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1784270.155056383926305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1239980.10775656988064569No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1223010.10628184529567289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT54803550.0132.482561
GTGGTAT55312800.0131.315876
GGTATCA55321700.0131.304758
TGGTATC55420150.0131.07977
AGCAGTG55522200.0130.781712
GTATCAA55565950.0130.761699
CAGTGGT55636250.0130.555474
AGTGGTA55886100.0129.958485
GCAGTGG57959600.0125.3177643
CATGGGG20914450.027.11574225-29
CAACGCA55106100.026.83283810-14
ATCAACG55227750.026.704510-14
TCAACGC55366400.026.70161810-14
CGCAGAG55177050.026.68860215-19
ACATGGG54365000.026.67160820-24
TACATGG55272500.026.64088220-24
AGAGTAC55425200.026.60268415-19
GTACATG55389150.026.59826320-24
GCAGAGT55487900.026.5806915-19
CAGAGTA55588950.026.54047615-19
Produced by FastQC (version 0.11.5)