FastQCFastQC Report
Mon 20 Mar 2023
SRR6211737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44623488
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT4267850.0143.144041
GCTGATC4275550.0142.886262
ACGTATC2670050.0142.297261
ACGCTTA1575300.0141.363782
GATAGAC1502900.0141.314291
CTGATCG1602350.0141.165393
GACGGAT999600.0141.108813
GTCACTA1108200.0140.857732
CGTGAGA1540350.0140.794952
AGACGGA2881050.0140.371662
ACTTCGA927050.0140.200532
AAGACGG2884650.0140.196491
GAGTCAA1174850.0140.060682
GAGCTGA1006250.0139.582461
AGAGTCA1179100.0139.555831
CCGTGAG1556850.0139.302761
CTGATCA1251050.0139.206193
ATAGACA1526550.0139.124982
AGTCACT1122450.0139.069471
GACGGAA876200.0138.419973
Produced by FastQC (version 0.11.5)