FastQCFastQC Report
Mon 20 Mar 2023
SRR6211736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86719651
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1301530615.00848521634387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13259941.529058275384434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5726410.6603359139441186No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1654240.19075722525682212No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1337090.15418535298302802No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1129990.13030379930841743No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1101060.1269677618974735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG996610.11492320235467737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC978240.11280488202149246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT964630.11123545688623676No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT949610.10950343884571215No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC901790.10398911776063305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99998700.055.5953331
AGCAGTG101478700.054.675022
GTATCAA101469500.054.619679
AGTGGTA101604650.054.555435
GGTATCA102498950.053.9491848
CAGTGGT102940900.053.809074
GTGGTAT103845350.053.468876
GCAGTGG103890050.053.4037673
TGGTATC103669000.053.390837
GGTATAA567550.029.829098
TGGTATA614100.027.6617877
TGACTGG675800.023.3351062
AGTTCAG699400.022.641439
TGGAGTT737750.021.6791446
ATGGGGT2127350.020.76073825-29
GACTGGA765850.020.6289353
ATGGGGG3545800.020.46850425-29
CATGGGG10400850.020.4378225-29
GTGACTG803400.019.9082551
ATGGGGC2086500.019.71483825-29
Produced by FastQC (version 0.11.5)