FastQCFastQC Report
Mon 20 Mar 2023
SRR6211735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62564680
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC5096500.0139.144672
CCTTACG1616900.0137.366993
TCACGCA3492850.0136.549562
CTAACGA2223550.0135.841432
GTCGTAG1483650.0135.462071
TAGCGAC888850.0135.328312
CCGACAA2985100.0135.220411
AACTCAC5509000.0134.629881
GCCAAGA4502500.0134.379071
TCGTAGA1431300.0134.195862
CCAAGAC4444600.0134.083282
CACCTTA5396900.0134.052631
CGACAAC2960400.0133.840552
ATAGCGA910000.0133.774341
ACTCACC5403100.0133.384752
CACGCAT1246650.0132.364563
ATCCGTC899100.0132.138092
TAGGATG3144650.0130.74021
CAAGACT1760750.0130.30143
AGAGATC2897200.0130.113892
Produced by FastQC (version 0.11.5)