FastQCFastQC Report
Mon 20 Mar 2023
SRR6211733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6211733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96024969
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG979330110.198702589531687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10336221.0764096159197927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5318340.5538496971553305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGACCTCGGATCAGGTAG4059540.4227587930801623No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTAAGTGAATAAGGGCGCAC4008280.4174205981779593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1676780.1746191659796318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA1584330.16499146175199494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1404810.1462963242404171No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC1349720.14055927474446775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCAAGTGAAGAAGCGCAT1281920.13349861117893203No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1197850.12474359663682891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1133520.11804429741601895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1113570.11596671278279716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGGGCTTGGCCTTGAGA985340.10261289436084067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT77067100.058.4667131
GTATCAA77997800.057.648199
AGCAGTG78158500.057.6284262
AGTGGTA78411500.057.4778065
GGTATCA78806200.057.009168
CAGTGGT79161800.056.884064
GTGGTAT79889650.056.3973126
TGGTATC79888800.056.3145877
GCAGTGG81048850.055.592393
GGTATAA370000.022.9309678
ATGGGGT2220250.022.92061825-29
AGTAACG604450.022.551529110-114
TACCCGC625450.022.2407750-54
TCGGATC642350.022.01469435-39
CGCTGAA652700.021.76109955-59
GTTGACC659800.021.5890830-34
ATGGGGC2000200.021.57385325-29
CTCGGAT659700.021.42698535-39
TAAGCGG653200.021.3884975-79
GCGATAA481100.021.3182995-99
Produced by FastQC (version 0.11.5)