FastQCFastQC Report
Mon 20 Mar 2023
SRR6155081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108342749
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1196080.11039778951889065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3117550.0141.244771
AGTTAGC3392950.0140.261462
TAGCGAC2711750.0139.441212
GAGTTAG3974450.0138.271381
GGCTTCA6693050.0137.99812
ATCATTC5231100.0137.911381
ATGAATC2937100.0137.412632
TCACGCA2754900.0137.33032
TGGCTTC8133150.0137.24321
CACCTTA8348650.0136.979451
CAGATTC3167550.0136.588912
GATGAAT3452150.0136.397641
AGAGATC3302450.0135.47862
TTCACGC3373600.0135.371281
ACCTTAC7284250.0134.933322
ATCCGTC2416500.0134.651542
ACAGATT4037600.0134.409331
GCTTCAG2269350.0134.20773
CCTAATC2588550.0133.659061
TAAGGTC1791000.0132.840682
Produced by FastQC (version 0.11.5)