FastQCFastQC Report
Mon 20 Mar 2023
SRR6155081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108342749
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1608784914.849031567400973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG28322062.6141167970548724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8125970.7500243509604874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5057650.4668194269281463No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4421770.4081279126487735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3640460.33601325733390797No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2522640.23283884000395821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2238700.20663127165067596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2232730.20608024261965147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2112350.19496920832237696No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2033040.18764892147973836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT1800090.16614771330936046No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1695720.1565143967318016No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1678200.15489730651010156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1655240.1527781060825769No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1577300.1455842697880963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1548660.14294080723390173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1523270.14059731860781935No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1511290.1394915685589628No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1508340.13921928453190716No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1489510.1374812817422604No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1454700.13426833022300366No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1450030.13383729076322404No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1415580.1306575671252351No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1414430.1305514225045185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1397800.12901647898928614No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1386080.12793472685467855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1293720.11940992931608187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1278330.11798943739188304No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1268830.11711259052509365No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1254920.11582870211277362No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1194190.11022334314223464No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGTCGCCAGGAGGAG1142570.10545883416711163No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA1127430.10406141716045991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT117678750.055.74741
AGCAGTG121345650.054.075912
AGTGGTA121242100.054.0160835
GTATCAA121670450.053.8036089
GGTATCA123290150.053.1135258
GTGGTAT123617450.052.9713336
TGGTATC123712400.052.948957
CAGTGGT123988600.052.873544
GCAGTGG124640300.052.645523
ATGGGGC2747750.020.60721825-29
CATGGGG13157250.020.07060225-29
CGTAAGT612350.019.65921230-34
GAAACCG651950.019.18117585-89
ATGGGGG4560650.019.17018725-29
TACGCAC202750.019.15466350-54
AAACCGC652350.019.14513285-89
GCCGTAA633000.018.9291630-34
TGAGTCG482700.018.82053870-74
TTAGGGC522250.018.7794745-49
ACCGTAC479550.018.59608850-54
Produced by FastQC (version 0.11.5)