FastQCFastQC Report
Mon 20 Mar 2023
SRR6155080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133030951
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG22907811.7219909974183374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17775431.3361875463101816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8653330.6504749409782089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4250820.31953616568523213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4082850.3069097807171205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC3900920.2932340158945417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2338190.17576285687080445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC2221190.1669679110991246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA2112280.15878109448379424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2037060.15312677122784757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1643630.12355245058723215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC1584560.11911213052968404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGTCGCCAGGAGGAG1563360.1175185164240463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1544520.1161023046433758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1352660.10168009698735447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT43655200.0117.772481
AGCAGTG44454450.0115.691492
GTGGTAT44472450.0115.458546
GGTATCA44479250.0115.4323048
GTATCAA44490900.0115.354669
TGGTATC44563800.0115.2507867
CAGTGGT44670800.0115.051444
GCAGTGG44859500.0114.698743
AGTGGTA44870300.0114.4352955
CATGGGG13198900.025.94940225-29
CAACGCA44297250.023.76707610-14
ATCAACG44403400.023.66320610-14
CGCAGAG44209750.023.63256815-19
CAGAGTA44190350.023.62622815-19
AGAGTAC44221300.023.6199515-19
TCAACGC44616350.023.57959710-14
ACATGGG43749400.023.4772320-24
GTACATG44448600.023.40369820-24
TACATGG44562350.023.38877720-24
AGTACAT44423300.023.3700620-24
Produced by FastQC (version 0.11.5)