FastQCFastQC Report
Mon 20 Mar 2023
SRR6155078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110646178
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG5443200.0139.88641
ATGCGTC3129300.0139.797643
CCGTGAG4829100.0139.301911
ATGCCTA2419950.0138.839371
CGTGAGA4160200.0138.771532
CTCAATG2452550.0138.338011
AATGCGT4234050.0138.254912
ACGTGAT2208350.0138.12852
CTGGCAT2600600.0137.257321
ACGCTTA2393050.0137.052672
AACGTGA2220600.0136.921481
CAAGCTA2614800.0136.828022
GACTAGT2725100.0136.802631
ACTAGTA2469450.0136.432392
CCGAAGT2255150.0136.426011
ACGTATC1803350.0136.176131
CGAAGTA2076900.0136.086522
TGGCATA2369600.0136.08542
GTCACTA1538250.0135.921772
AACATCG2395050.0135.712162
Produced by FastQC (version 0.11.5)