FastQCFastQC Report
Mon 20 Mar 2023
SRR6155077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132628945
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG10011600.0141.910081
TCGTAGA8329150.0141.301472
AGTTAGC5598350.0140.25082
GATAGAC4304950.0140.01611
TATCAGC3424000.0139.135911
GCGAGTA3446050.0138.114061
ATAGCGA2100000.0137.46031
ATCAGCA3024100.0137.05072
TAGCGAC2039750.0137.04842
ATAGACA3924500.0137.0092
GAGTTAG5946300.0136.98581
GCCACAT4077150.0136.825121
CGAGTAA2977300.0135.811052
CCGTCTA4481150.0135.342352
CGTAGAG2627350.0134.608443
ATGAATC2896250.0134.159582
TCTGTCA4351450.0134.14822
TCACGCA2176350.0133.562362
GTCTGTC5004950.0133.35451
GTTAGCG1826050.0133.01193
Produced by FastQC (version 0.11.5)