FastQCFastQC Report
Mon 20 Mar 2023
SRR6155076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115316269
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT4822400.0139.292371
ATGCCTA2679250.0139.09771
ACTTCGA3370700.0138.879752
GAGCTGA3654700.0138.7411
TGGCATA4326000.0138.70882
CCGTGAG4262950.0138.317861
CGACTGG6156300.0138.302751
CGTGAGA3645450.0137.871552
ACGTGAT2456550.0137.756742
GCTCATG2984800.0137.556991
CACTTCG3888650.0137.111041
GACTAGT2861300.0136.795471
AACGTGA2467800.0136.616791
AGCTGAA3288050.0136.427812
ACTAGTA2594500.0136.23272
CGCATAC2875250.0135.956531
CCGAAGT2587850.0135.944691
CGAAGTA2385000.0135.793692
CTCAATG2059600.0135.68021
ACGTATC1914550.0135.515931
Produced by FastQC (version 0.11.5)