FastQCFastQC Report
Mon 20 Mar 2023
SRR6155074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134912655
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT4353750.0139.629652
ATGCCTA2754650.0138.649811
CTACGTC3525350.0138.483873
AACGTGA4292300.0138.304051
ACGTATC2885750.0137.747181
CGACTGG4567750.0137.647461
CTCTACG4506150.0137.591161
TCTACGT4501600.0137.487242
CTCAATG2816850.0136.825241
AACATCG3423150.0136.17622
AGGTACA4780850.0136.02632
TACGTCC2762500.0135.98184
CAAGCTA3423000.0135.626942
AGCAGGA7493600.0135.242071
CTGGCAT2692500.0135.141541
CGCATAC2851850.0135.065251
ACCGAGA4183300.0134.998842
GTCACTA1976700.0134.95742
ACGCTTA2358450.0134.778442
AGTCACT2061150.0134.286931
Produced by FastQC (version 0.11.5)