FastQCFastQC Report
Mon 20 Mar 2023
SRR6155073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133368170
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC4232250.0139.580731
ATGCCTA2721350.0138.663531
GCGAGTA2960300.0138.468581
ACGTGAT3139550.0138.081512
ACGTATC2985900.0137.968581
CAAGCTA4082550.0137.64092
GCTAGTC2427050.0137.626343
ATCAGCA3434800.0136.907912
GCTAACG2373700.0136.751821
AACGTGA3162700.0136.609361
ATAGACA4095900.0136.552492
CGAGTAA2814300.0136.447772
CTAACGA2201600.0136.446562
GTCACTA2565600.0136.427192
GTCGTAG1748150.0136.331531
TATCAGC3697300.0135.823031
CAATGGA6687650.0135.587131
AGGTACA4425850.0135.478262
GCCACAT3131900.0135.185091
CCGTCTA2386650.0134.774672
Produced by FastQC (version 0.11.5)