FastQCFastQC Report
Mon 20 Mar 2023
SRR6155073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133368170
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG129017199.67376173790193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG33046172.4778153587921317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8780080.6583339937857736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7895230.5919875784454417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC5358030.40174728347850913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAGTTGAGTGCTGAAAAA4710630.35320496637241106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2990160.22420342125111262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2854190.2140083349722801No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2804190.21025931449760463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2801270.2100403717018836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2564110.19225801778640286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1714480.1285524124684323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1652430.12389987805936005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1508720.11312444341104778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATGTCGCATATAGGACT1452580.10891504322208215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1401900.10511503606895109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1367670.10254845665198825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT110023400.069.647011
AGCAGTG112962900.067.861632
AGTGGTA113003300.067.78635
GTATCAA114195700.067.08279
CAGTGGT114316750.067.079184
GCAGTGG114550400.066.934183
GGTATCA115438450.066.387538
GTGGTAT115496950.066.322746
TGGTATC115714950.066.239597
GACTGGA831700.026.0788273
TGACTGG880600.024.6879022
AGTTCAG941100.022.8578459
CATGGGG16069500.022.48808525-29
GTGACTG996850.021.8811041
ACTGGAG993300.021.8175284
ATGGGGC3388350.021.7206625-29
CTGGAGT1021350.021.2027325
TGGAGTT1053650.020.6279286
ATGGGGG6421750.020.60224225-29
ATGGGGT3449550.019.99326325-29
Produced by FastQC (version 0.11.5)