FastQCFastQC Report
Mon 20 Mar 2023
SRR6155072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96050735
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG5590150.0140.474581
TACCGTC3359600.0140.381133
TTACCGT4874800.0139.545852
GACTAGT3662000.0139.510351
ACGTGAT3066750.0139.43272
GAGCTGA3467650.0139.412081
ATGCCTA1874500.0139.067211
CTCAATG2489850.0138.595781
ACTAGTA3386150.0138.538672
ACGTATC1951400.0137.733611
AACGTGA2974500.0137.72361
AGTCACT1734250.0137.34491
AGCTGAA3180900.0137.222612
ACGCTTA2218800.0137.191482
GTCACTA1665000.0136.562642
ACTTCGA1496100.0136.148762
CGAACTT2414200.0135.974811
CGCATAC2504550.0135.627461
CTGGCAT1626000.0134.558141
TGCCTAA1872250.0134.138432
Produced by FastQC (version 0.11.5)