FastQCFastQC Report
Sun 19 Mar 2023
SRR6155071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96334222
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA3841700.0139.801472
ACGTATC2016050.0137.555631
AGCAGAT3615900.0136.829153
ACCGAGA4369850.0136.70952
GTCGTAG1396150.0136.709431
CCGAGAT1951450.0136.371143
AGTCACT4135900.0136.021581
GATAGAC1554800.0135.771821
CAAGCTA2790950.0135.685442
AACCGAG4951150.0135.661991
ACAGCAG8166400.0135.466111
ACGTGAT1556950.0135.292272
GCGAGTA1373550.0135.22391
GCTAACG1459850.0134.550261
CTAACGA1324200.0134.377352
ATGCCTA1116200.0133.698211
CAATGGA3682750.0133.097721
TCGTAGA1296550.0132.860842
CAGCAGA7671350.0132.859592
ACGCTTA1488350.0132.354462
Produced by FastQC (version 0.11.5)