FastQCFastQC Report
Sun 19 Mar 2023
SRR6155070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104846791
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1951040.18608485594947777No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1119160.10674241808697799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA2958550.0140.591641
CTTAGTC4005800.0140.390323
GTCACTA3194700.0139.755172
CCTTAGT5727500.0139.478582
ACCTTAG5611150.0139.440981
CTCAATG2229600.0138.27211
ACGTGAT2170800.0138.125152
AACGTGA2166650.0137.65021
ACGTATC1895550.0137.208441
TAGTCCC3265450.0136.800955
AGAGTCA1677350.0136.566971
TGCCTAA2903300.0136.539292
TTAGTCC3112550.0135.966024
AACATCG2445600.0135.633992
CAAGCTA2085900.0135.541172
TAGTCAC1202750.0135.360645
GAGTCAA1612800.0134.11222
AGATCGC2430750.0133.997251
CTGTAGC1493700.0133.416471
TAGTGCC1129000.0133.088245
Produced by FastQC (version 0.11.5)