FastQCFastQC Report
Sun 19 Mar 2023
SRR6155069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137893046
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2141066515.527008519341868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG37100052.690494631614708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA19594251.420974484819198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12253470.8886213159726706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7247210.5255674749544659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5564350.40352651285982905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC5169940.37492391023112215No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4735850.34344371506595045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4206350.30504438925803407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3051910.22132443140026073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2746040.1991427471984338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2642900.19166303716287475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCATCTCATCCCCGCCTTGT2587750.1876635606410493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2538790.18411298275331447No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2059790.14937591559185662No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1737750.12602158342343092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1699650.12325857244461769No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA1676900.12160874305438144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1502220.10894095413629487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGTAAAAAAAAAAAA1482020.10747605067771147No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1387670.10063379120655584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTAAGTTAGAAAAAAAAA1383920.10036184130706635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT171503950.070.884621
AGTGGTA175867400.069.185515
AGCAGTG176130600.069.102792
GTATCAA177573900.068.5490349
CAGTGGT178190650.068.328034
GCAGTGG178817750.068.081083
GGTATCA179113150.067.974938
TGGTATC179675900.067.786457
GTGGTAT179594550.067.78446
CATGGGG23739850.022.74351325-29
ATGGGGC3971300.022.43288825-29
ATGGGAT10316700.022.10263425-29
ATGGGGG7203650.021.6357525-29
ATGGGGT5591150.021.57664925-29
ATGGGAG10221450.020.9926925-29
TGGGGAA4529850.020.4917125-29
ATGGGGA9168750.020.35541225-29
GGGGAAA4779800.020.0657325-29
TGGGATT4144150.020.03405625-29
ATGGGAC6135100.019.83519225-29
Produced by FastQC (version 0.11.5)