FastQCFastQC Report
Sun 19 Mar 2023
SRR6155068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100069519
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3847990.3845316774231722No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2046100.2044678559911935No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1217170.12163244234240798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA2570600.0140.075421
ACGTGAT2222450.0138.198012
AACGTGA2246750.0137.185961
GTCACTA1567550.0136.51942
ACGCTTA2503800.0136.353642
TGCCTAA2486100.0136.206542
ACTTCGA1399250.0135.887572
CGAACTT1821600.0135.784061
CTCAATG1290550.0134.849171
AACATCG2218600.0134.7612
CAAGCTA1765350.0134.42892
GCCTAAT1026100.0134.033163
CCGAAGT1798900.0133.258261
ACTAGTA1617200.0133.147262
CTGGCAT1222250.0133.087521
GACTAGT1760950.0132.576451
CGAAGTA1689500.0132.27772
AGTCACT1781500.0131.815161
AGTAGGC1441100.0131.77153
CTATGCA877250.0131.579252
Produced by FastQC (version 0.11.5)