FastQCFastQC Report
Sun 19 Mar 2023
SRR6155067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120753622
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC4366800.0140.729322
ATAGCGA4630050.0140.391951
TATCAGC3610350.0139.243931
GGCTAAC5164150.0139.147922
AGGCTAA5512700.0137.095721
GTCGTAG2420200.0136.866071
GATAGAC2530300.0136.830111
ATCATTC5753250.0136.725571
ATCAGCA3287000.0136.572312
GCGAGTA2735650.0134.72431
TCGTAGA2163300.0134.107592
GCTAACT1761900.0134.086063
CAGATTC3074550.0133.886252
AGTTAGC1809800.0133.676012
CTCGGTA2079750.0133.60652
TCATTCC5548200.0133.198822
CATTCCG1732400.0133.081973
CGAGTAA2472550.0132.790572
AGCGACT1446900.0132.207373
TAGGATG3907450.0131.86321
Produced by FastQC (version 0.11.5)