FastQCFastQC Report
Sun 19 Mar 2023
SRR6155066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106566340
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3356760.3149925201522357No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3269350.30679011778015464No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1318760.12375014474551721No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCGGCGTTGATAC1123840.1054591909602976No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATAC1082750.10160337682611602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3166250.0140.052261
ACGTATC2871450.0139.155781
ACGTGAT2594350.0138.683642
AACGTGA2601250.0137.363161
CAAGCTA2814900.0137.084322
GTGTATG6244200.0136.192761
TGCCTAA3038200.0136.163312
CGAACTT1995350.0136.070511
AGTCACT1971250.0136.04271
GTATGTC2081000.0135.951373
GTCACTA1792900.0135.815322
AACATCG2599700.0135.800492
ACGCTTA2245200.0134.923482
ACAAGCT3092350.0134.869281
CTCAATG1416000.0134.734851
GCCTAAT1254350.0134.128543
ACTTCGA1060250.0133.157472
GTATGGC1290400.0132.06083
TATGTCC1481200.0131.97954
AGGTACA2892800.0131.633182
Produced by FastQC (version 0.11.5)