FastQCFastQC Report
Sun 19 Mar 2023
SRR6155064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110295571
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG106316319.63921842337622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7154180.6486371061989424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6141380.5568111161961345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCGGGGACGGGTGGCGTGTG6007180.5446438098588745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5825660.5281862133883871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3082000.27943098458595406No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2172350.19695713801599524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGTAAGTTAGAAAAAAAA2160180.19585373922222138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2093490.18980725889709568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCCATCGGCTGGCACCAC1887880.1711655312070509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGAGCCTCTCTCCCTAC1492540.13532184352171311No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGCGTCGCCAGGAGGAGC1442050.130744143842367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1366400.1238853008884645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1360500.12335037460389049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1245400.11291477878109901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1170450.10611940165756974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT92114950.071.472121
GTATCAA93951700.070.090349
AGCAGTG93975250.070.0503852
AGTGGTA94237800.069.856225
CAGTGGT94914550.069.371034
GGTATCA95063400.069.262588
GTGGTAT95536400.068.887486
TGGTATC95663700.068.819297
GCAGTGG95649250.068.809483
GACTGGA733300.031.7616123
TGACTGG764400.030.6672882
GTGACTG770800.030.2636031
ACTGGAG922700.025.461214
AGTTCAG935350.024.8780849
TGGAGTT959400.024.3820256
CATGGGG13407300.023.72866625-29
CTGGAGT989000.023.55785
AAACCGC1291550.023.45777785-89
GAAACCG1316300.023.19174685-89
ATGGGGG4642200.022.62941725-29
Produced by FastQC (version 0.11.5)