FastQCFastQC Report
Sun 19 Mar 2023
SRR6155063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6155063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114659929
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC4073650.0139.791281
ATAGCGA3030200.0138.920751
TAGCGAC2911300.0138.90912
ATCAGCA3790650.0137.481582
GATAGAC2554400.0137.331361
AGTTAGC2246950.0136.16782
TCACGCA2382450.0135.346122
GGCTAAC2542400.0134.878942
GTCTGTC2974450.0134.289551
ATGAATC2508850.0133.331532
ATCATTC3347000.0133.081381
ATCCGTC1762400.0132.561922
TAAGGTC2128500.0132.355872
GCCACAT2585300.0132.338211
CAGATTC2212650.0132.15262
GCGAGTA1689150.0132.053671
ATAGACA2475250.0131.470462
ACCTTAC2787550.0131.429182
TTCACGC2708200.0131.324781
TCTGTCA2760600.0130.943472
Produced by FastQC (version 0.11.5)